Sigma-Aldrich is a global supplier of leading RNA interference (RNAi) tools including siRNA through Sigma-Proligo and the comprehensive MISSIONTM shRNA library through participation in The RNAi Consortium (TRC).
Introduction to TRC and the MISSION shRNA Library
The RNAi Consortium (TRC) is a collaborative group of 11 world-renowned academic and corporate life science research groups whose mission is to create comprehensive tools for functional genomics research and make them broadly available to scientists worldwide. As a member of TRC, Sigma-Aldrich is both a collaborator and supplier of the TRC shRNA libraries (MISSION libraries). The collection will consist of 150,000 pre-cloned shRNA constructs targeting 15,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,000 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated silencing. The current release of the collection, which is being developed by TRC at the Broad Institute of MIT and Harvard, includes approximately 117,156 clones targeting 12,037 human and 12,615 mouse genes. Additional releases will be made on a quarterly basis. Sigma-Aldrich is committed to providing unmatched infrastructure and service for RNAi. With the addition of state-of-the-art robotics, liquid handling, LIMS, and dedicated laboratories in our new Life Science & High Technology Center, our goal is to provide superior quality and convenient service for your RNAi research needs.shRNA Design
MISSION shRNA clones, designed and developed by TRC at the Broad Institute of MIT and Harvard, include hairpin sequences comprised of a 21 base stem and a 6 base loop. Rules based design consisting of sequence, specificity, and position scoring are utilized to generate optimal shRNA sequences. The hairpin sequences are each cloned into the pLKO.1 vector and sequence verified. Typically, 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. One in five clones will typically provide at least 70% knockdown of the gene target. Numerous target sets include a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs.Visit www.sigmaaldrich.com/rnai for more information!